The injection only works on Subjects of Ymir and has no effect on other living things. While the exact chemical composition of Titan serum is currently unknown, it is considered by the Marleyans to be Titan spinal fluid. It has been shown that the serums quickly evaporate after coming into contact with air; for this reason, they are always kept in tightly-sealed vials. Since Titan corpses also quickly evaporate, it would make sense that their spinal fluid would do the same.
There are different types of serums: Rod Reiss had with him a "strongest" and an "armor" serum along with the regular serum. It is unknown if there are more types. The "strongest" serum turned Rod into a 120 m Titan, although he claimed that it makes a Titan most suited for battle, and the "armor" serum gave Eren Yeager's Titan hardening abilities. The size of the resulting Titan can also be controlled, as Sergeant Major Gross claimed he could turn an Eldian captive into a three or four meter Titan, and then successfully did it.
Titan serums are injected into the bloodstream, typically the inner forearm or the back of the neck; it can also be orally taken in. Very shortly after this, the person injected generates a Titan body and becomes a "Pure Titan". Pure Titans are mindless until they consume an human with the power of the Titans. Humans with the power of the Titans can take the serum again, and specialized serums can give the Nine Titans abilities they never had; it is unknown what would happen if they take the normal serum.
Marley's Public Security Authorities use the injection as a life sentence against Eldians who break the law, including the Eldia Restorationists, to roam Paradis as mindless Titans for the rest of eternity.
On the night of the fall of Shiganshina, Grisha Yeager traveled to the Reiss Chapel to speak with Queen Frieda. His reasons were presumably unknown to the Reiss family. After battling with Frieda in her Titan form and slaughtering most of the Reiss family, he reunited with his son Eren Yeager and injected him with a serum, transforming him into a Titan.
Five years later, Rod Reiss prepares to use another serum for his illegitimate daughter Historia. Upon seeing the Titan injection syringe prepared by Rod Reiss for Historia, Eren notes the identical embellished design of the syringe to the one used on himself years ago, indicating that Grisha had stolen an injection from the Reiss family. After much discussion and contemplation, Historia ends up shattering the syringe and chooses to free Eren, who was the prepared sacrifice for Historia's mindless Titan. In desperation, Rod chooses to lick the exposed serum, becoming a monstrous mindless Titan. In order to save his comrades from the collapse of the Chapel's caverns, Eren bites into an injection vial labeled "Armor", granting him the ability to harden his Titan.
At some point during the ritual for Historia's transformation, Kenny Ackerman steals another injection from Rod Reiss' bag. As he is dying, he is found by Captain Levi. Just before his death, he hands the injection over to Levi. It is later decided by the veterans of the military that Levi should be entrusted with the injection. This injection later becomes a subject of conflict as both Armin Arlert and Erwin Smith are brought to Levi on the brink of death. Ultimately, Levi chooses to save Armin with the injection, and Armin's mindless Titan consumes Bertolt Hoover, the Colossus Titan, and Armin inherits the power of the Titans.
- While only the "Armor" vial read by Eren has been identified officially in the story, the labels of the three Titan injection vials that have been introduced each bear legible katakana:
- "Strongest Titan" (サイキョウノキョジン Saikyō no Kyojin?) - The one Rod chose for Historia and which he ended up ingesting.
- "Armor Braun" (ヨロイ ブラウン Yoroi Buraun?) - The one Eren ingested and which granted him hardening abilities.
- "Normal Titan" (フツウノキョジン Futsū no Kyojin?) - The one Kenny gave to Levi and which was injected into Armin.